effects of local administration of platelet rich plasma on functional recovery after bridging sciatic nerve defect using silicone rubber chamber; an experimental study
نویسندگان
چکیده
objective: to determine the effects of local administration of platelet rich plasma (prp) on peripheral nerve regeneration in rat sciatic nerve transection model. methods: forty-five male white wistar rats were randomized into three experimental groups (n = 15): normal control group (nc), silicon group (sil), prp treated group (sil/prp). in nc group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. in sil group left sciatic nerve was exposed the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. proximal and distal stumps were each inserted into a silicone conduit and filled with 10 µl phosphate buffered solution. in sil/prp group silicon conduit was filled with 20 µl prp. each group was subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks after surgery. results: the animals were comparable regarding the baseline characteristics. behavioral testing, sciatic nerve functional study and gastrocnemius muscle mass showed earlier regeneration of axons in sil/prp than in sil group. conclusion: local administration of prp combined with silicon grafting could accelerate functional recovery of peripheral nerve. easily available growth factors and bioactive proteins present in prp may have clinical implications for the surgical management of patients after nerve transection.
منابع مشابه
Effects of Local Administration of Platelet Rich Plasma on Functional Recovery after Bridging Sciatic Nerve Defect Using Silicone Rubber Chamber; An Experimental Study.
OBJECTIVE To determine the effects of local administration of platelet rich plasma (PRP) on peripheral nerve regeneration in rat sciatic nerve transection model. METHODS Forty-five male white Wistar rats were randomized into three experimental groups (n=15): Normal control group (NC), silicon group (SIL), PRP treated group (SIL/PRP). In NC group left sciatic nerve was exposed through a glutea...
متن کاملBridging Small-Gap Peripheral Nerve Defect Using Silicone Rubber Chamber in the Rat Sciatic Nerve Transection Model
Despite promising results observed using silicone rubber chamber, no previous comprehensive work was performed on behavior of the conduit. Present study aimed at further functional, histomorphometrical and immunohistochemical assessment of nerve regeneration in the same animal along a 10-mm rat sciatic nerve gap. Fifty- four male Wistar rats were divided into three experimental groups (n = 1...
متن کاملbridging small-gap peripheral nerve defect using silicone rubber chamber in the rat sciatic nerve transection model
despite promising results observed using silicone rubber chamber, no previous comprehensive work was performed on behavior of the conduit. present study aimed at further functional, histomorphometrical and immunohistochemical assessment of nerve regeneration in the same animal along a 10-mm rat sciatic nerve gap. fifty- four male wistar rats were divided into three experimental groups (n = 18),...
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The repair of peripheral nerve injuries is still one of the most challenging tasks and concerns in neurosurgery. Effect of methylprednisolone-laden hydrogel loaded into a chitosan conduit on the functional recovery of peripheral nerve using a rat sciatic nerve regeneration model was assessed. A 10-mm sciatic nerve defect was bridged using a chitosan conduit (CHIT/CGP-Hydrogel) filled with CGP-h...
متن کاملeffect of local administration of platelet-derived growth factor b on functional recovery of peripheral nerve regeneration: a sciatic nerve transection model
background: effects of platelet-derived growth factor b (pdgf-b) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model. materials and methods: forty‑five male, white wistar rats were divided into three experimental groups (n = 15), randomly: normal control group (nc), silicon group (sil), and pdgf‑b treated group (sil/pdgf). in nc group, left sciatic nerve was...
متن کاملThe Healing Effect of Silicone Gel on Sciatic Nerve Injuries in Experimental Rat
BACKGROUND Peripheral nerve repair is often complicated by fibroblastic scar formation, nerve dysfunction, and traumatic neuroma formation. Use of silicone may improve outcomes of these repairs. In this study, we tried to evaluate effectiveness of silicone gel on rats’ sciatic nerve repair, axon regeneration and scar formation around and in the nervous tissues. METHODS This experimental stud...
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عنوان ژورنال:
bulletin of emergency and traumaجلد ۳، شماره ۱ JAN، صفحات ۱-۷
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